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Details of the primary and secondary antibodies used for immunohistochemical staining. |
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| Host |
Dilution |
Source |
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| Primary Antibodies – Transporters |
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| α1 Na+/K+ ATPase (clone 05–369) |
Mouse |
1:100 |
Millipore, Australia. |
| α3 Na+/K+ ATPase (clone XVIF9-G10) |
Mouse |
1:400 |
Biomol, PA, USA. |
| NKCC1 |
Rabbit |
1:1000 |
Gift Dr RJ Turner, National Institute of Dental and Craniofacial Research, USA. |
| vGLUT1 (catalogue# 135 302) |
Rabbit |
1:2000 |
Synaptic Systems Goettingen, Germany. |
| vGLUT2 (catalogue# 135 402) |
Rabbit |
1:2000 |
Synaptic Systems Goettingen, Germany. |
| Primary Antibodies – Neurotransmitters |
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| CGRP (catalogue# RPN 1842) |
Rabbit |
1:4000 |
Amersham, UK. |
| Neuronal nitric oxide synthase (nNOS) |
Sheep |
1:4000 |
Gift Dr Colin Anderson, University of Melbourne, Australia. |
| Somatostatin (catalogue# AB5494) |
Rabbit |
1:100 |
Millipore, Australia. |
| Substance P (clone NC1) |
Rat |
1:200 |
Millipore, Australia. |
| Primary Antibodies – Cytosolic Proteins |
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| Calbindin D28k (number CB-38A) |
Rabbit |
1:1000 |
Swant Bellinzona, Switzerland. |
| Calretinin (number 7699/4) |
Rabbit |
1:1000 |
Swant Bellinzona, Switzerland. |
| Neurofilament 160KD (clone NN18) |
Mouse |
1:400 |
Millipore, Australia. |
| Parvalbumin (number 235) |
Mouse |
1:400 |
Swant Bellinzona, Switzerland. |
| Secondary Antibodies (IgG, H+L, 2 mg/ml) |
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| AlexaFluor 488 or 594 anti-goat |
Donkey |
1:200 |
Molecular Probes Eugene, OR, USA |
| AlexaFluor 488 or 594 anti-mouse |
Donkey |
1:200 |
Molecular Probes Eugene, OR, US |
| AlexaFluor 488 or 594 anti-rabbit |
Donkey |
1:200 |
Molecular Probes Eugene, OR, USA |
| AlexaFluor 488 anti-rat |
Goat |
1:200 |
Molecular Probes Eugene, OR, USA |
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Note, AlexaFluor 488 and 594 are green and red fluorphores, respectively. | |||
Mazzone and McGovern Cough 2008 4:9 doi:10.1186/1745-9974-4-9 |
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